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| Gomes,ML; Hatanaka,T; Campos,WN de; Wasko,AP. |
| Microsatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, H E = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coturnix japonica; Parentage testing; Polyandry; Quail; SSRs. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400007 |
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| Gouvêa,Lígia Regina Lima; Rubiano,Luciana Benchimol; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Gonçalves,Paulo de Souza. |
| Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genealogy; Genetic diversity; Hevea brasiliensis; Multivariate analysis; SSRs. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200018 |
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| Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol. |
| A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
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| Bassi,Denis; Briñez,Boris; Rosa,Juliana Santa; Oblessuc,Paula Rodrigues; Almeida,Caléo Panhoca de; Nucci,Stella Maris; Silva,Larissa Chariel Domingos da; Chiorato,Alisson Fernando; Vianello,Rosana Pereira; Camargo,Luis Eduardo Aranha; Blair,Matthew Wohlgemuth; Benchimol-Reis,Luciana Lasry. |
| Abstract Angular leaf spot (ALS) and powdery mildew (PWM) are two important fungi diseases causing significant yield losses in common beans. In this study, a new genetic linkage map was constructed using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), in a segregating population derived from the AND 277 x SEA 5 cross, with 105 recombinant inbred lines. Phenotypic evaluations were performed in the greenhouse to identify quantitative trait loci (QTLs) associated with resistance by means of the composite interval mapping analysis. Four QTLs were identified for ALS resistance. The QTL ALS11AS, linked on the SNP BAR 5054, mapped on chromosome Pv11, showed the greatest effect (R2 = 26.5%) on ALS phenotypic variance. For PWM resistance,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudocercospora griseola; Erysiphe polygoni; Quantitative inheritance; SSRs; SNPs. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100109 |
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| Schuck,Mariane Ruzza; Biasi,Luiz Antonio; Mariano,Ada Michele; Lipski,Bernardo; Riaz,Summaira; Walker,Michael Andrew. |
| The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross-pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and' Roanoke', and between' Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscadinia rotundifolia; Vitis labruscana; Hybrid identification; Interspecific crosses; SSRs. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100009 |
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| Nazareno,Alison Gonçalves; Pereira,Rodrigo Augusto Santinelo; Feres,Juliana Massimino; Mestriner,Moacyr Antônio; Alzate-Marin,Ana Lilia. |
| Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ecological genetics; Ficus citrifolia; Ficus eximia; Moraceae; SSRs. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021 |
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